Charleroi, , Belgium
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DNAVision has developped a high throughput low cost method to quantify the expression of the miRNAs from classical total RNA extraction without genomic DNA interferences. More than 150 human miRNAs, and more than 100 mouse miRNAs have been validated using as little as 15 ?g of total RNA. This method assumes accurate and sensitive miRNAs expression profiling to identify specific miRNAs signatures associated with tissues, biological process or diseases.
MicroRNAs (miRNAs) are small, RNA molecules encoded in the genomes of plants and animals. These highly conserved, ~21-mer RNAs regulate the expression of genes by binding to the 3'-untranslated regions(3'-UTR) of specific mRNAs. Initially expressed as part of transcripts termed primary miRNAs (pri-miRNAs), they are digested in the nucleus to release Pre-miRNAs which appear to be approximately 70 nt RNAs with 1–4 nt 3' overhangs , 25–30 bp stems, and relatively small loops (Lee 2002, 2003). Then they are exported to the cytoplasm and processed to generate mature miRNAs (Lee 2003, Yi 2003, Lund 2003, Hutvagner 2002, Schwarz 2003, Khvorova 2003).
Virtually all of the miRNAs that have been studied in animals reduce steady state protein levels for the targeted gene(s) without impacting
The corresponding levels of mRNA (Olsen 1999). The mechanism by which miRNAs reduce protein levels is not fully understood.
Moreover, it has been shown recently that the miRNAs often do not act alone. Indeed, observations made by two laboratories is that mRNAs containing multiple, non-overlapping miRNA binding sites are more responsive to miRNA-induced translational repression than those containing a single miRNA binding site (Doench 2003, Zeng 2003). These observations coupled with the predictions that many mRNAs have target sites for many different miRNAs suggests that the expression of miRNA target genes can be fine-tuned in animals and they could greatly influence the gene expression.
It has been estimated that the miRNAs could affect 1-10% of the genes.
All these data and the fact that If miRNAs indeed regulate the translation of, but not the stability of target mRNAs, this might at least partially explainwhy gene expression profiles based on mRNA analysis do not always correlate with protein expression data (Kern 2003).
Several research groups already provided evidences that miRNAs may act as key regulators of processes as diverse as early development (Reinhart 2000), cell proliferation and cell death (Brennecke 2003), apoptosis and fat metabolism (Xu 2003), and cell differentiation (Dostie 2003, Chen 2003). Recent studies of miRNA expression implicate miRNAs in brain development (Krichevsky 2003), chronic lymphocytic leukemia (Calin 2002), colonic adenocarcinoma (Michael 2003), Burkitt’s Lymphoma (Metzler 2004), and viral infection (Pfeffer 2004) suggesting possible links between miRNAs and viral disease, neurodevelopment, and cancer.
We provide specialised in molecular biology, offers a wide range of genetic services and products to the pharmaceutical, food and biotechnology industries. Objectives :- Different applications are possible :- Discovery of new drug targets Finding new drug mechanisms to combat disease Confirmation of expected drug mechanisms Choice of best candidate compound based on optimal expression profile Identification of therapeutic targets Selection of candidate molecules Clinical trials - classification of patients Technologies :- Gene expression services : DNAVision provides you with a complete gene expression profiling programme from the Affymetrix GeneChip? product line, NimbleGen technology and quantitative real-time PCR using Applied Biotechnology ABI 7900 technology. Affymetrix Genechip? Services :- DNAVision has a service provider agreement with Affymetrix, Inc. For the GeneChip? series of products. GeneChip?-based genome-wide expression profiling is available to both academic and industrial customers. We provide a complete service program based on the Affymetrix GeneChip?, giving our customers access to the latest technologies, without them having to invest heavily in resources. We provide a comprehensive range of support - from guidance in study design, experiment planning and laboratory work, to data analysis and mining, and preparation of publications. Custom arrays :- DNAVision provides a complete custom array service based on Affymetrix GeneChip? technology (NimbleExpress Array Program or Custom Array Program). We can also help you in the use of the NimbleGen technology. Starting from a list of genes, Genbank accession numbers, pathways, gene ontologies, chromosomal locations, etc. On any species, we offer a wide range of bioinformatics support to help you design your own array. We assist you in the choice of genes and in the complete building of your array (controls, spikes, housekeeping genes, number and length of probes, etc.) Quantitative Real Time PCR expression profiling :- QPCR Validation of Microarray Data :- Real-time PCR is the gold standard for validating data generated from microarrays and for quantification of specific transcript levels, whenever quantitative data, reproducibility and comparability between several projects are required. This technology can be used either to repeat and validate data from microarray experiments or to do experiments based solely on Real-time PCR as expression profiling. Using ABI?s new 384-MicroFluidic Card (running on an upgraded ABI Prism 7900 HT) in combination with ABI?s validated Assays-on-Demand, DNAVision is now able to offer this service at a fraction of standard operating times and costs. This method is more reliable, less expensive and completely replaces conventional low density hybridisation arrays. Using the new 384-MicroFluidic Card, 12 to several hundreds of signals generated from microarray experiments can now be affordably validated! MicroRNA QUANTIFICATION :- DNAVision has developped a high throughput low cost method to quantify the expression of the miRNAs from classical total RNA extraction without genomic DNA interferences. More than 150 human miRNAs, and more than 100 mouse miRNAs have been validated using as little as 15 ?g of total RNA. This method assumes accurate and sensitive miRNAs expression profiling to identify specific miRNAs signatures associated with tissues, biological process or diseases. Toxicogenomics :- DNAVision has a high throughput, low density, highly flexible microarray design useful in toxicogenomics :- Approximately 900 genes involved in toxicogenomics. The choice of genes is based on the latest information from databases of hundreds of toxicology microarray papers and on the KEGG/ Genemap/GO and ABI TaqMan probe databases. 50 genes used as controls (spikes, housekeeping genes, etc.). Highly flexible system with 200 empty slots available, depending on your own design (type of tissue, pathways, new targets found in other experiments, etc.) High throughput system of 12 samples per chip Based on Nimblegen technology (results validated with Affymetrix technology) 12 short oligonucleotides (24 mer) or 1 long oligonucleotide (60 mer) per transcript. Based on rat and human genomes but easily adaptable to the mouse, dog, etc. BIOINFORMATICS services :- DNAVision uses a variety of statistic and bioinformatic tools to analyse small, large and/or complex datasets obtained from multiple microarrays. By combining different methods, we provide customers with meaningful, reliable data related to biological phenomena or pathways. We also analyse data generated from different platforms, using a variety of normalisation methods (median normalisation, rma, gcrma …).
Rue Adrienne Bolland 8
Charleroi - B-6041 () Belgium